Ahmad Ramazani S.A
Sharif University of Technology
Iran
Title: Fabrication of new polymeric nanocomposite scaffold for neural tissue engineering
Biography
Biography: Ahmad Ramazani S.A
Abstract
In this study, we reported new conductive polymeric nanocomposite scaffold for neural tissue engineering applications. The scaffolds was composed of chitosan/gelatin as biodegradable part and polyaniline/graphene (PANi/Gr) as nanocomposite as conductive part. PANi/Gr nanocomposite was synthesized via emulsion polymerization of aniline monomer in the presence of chemically reduced graphene nanosheets. The effect of electrical stimulation on morphology and growth of cultured Schwann cell on this conductive scaffold was investigated. The results demonstrated that showed that pulsed electrical stimulation with voltage of 100 mV/mm and time step of 1 sec was more effective on proliferation of cells compare to direct electrical stimulation as well as pulsed one with larger time steps. Furthermore, the SEM images showed that the electrical stimulated cells illustrated needle shape with significant extension of neuritis compare to non-stimulated ones which more confirmed the positive effects of using electrical stimulation.