Touba Eslaminejad
Pharmaceutical research center KermanM
Iran
Title: Cationic ẞ-cyclodextrin-chitosan conjugates as potential carrier for pmcherry-c1 plasmid delivery into u87 human glioblastoma cell line
Biography
Biography: Touba Eslaminejad
Abstract
Cationic ẞ-cyclodextrin-chitosan conjugates were prepared for carrying pmCherry-C1 plasmid in the form of CS/CD/TPP/pmCherry-C1 to achieve high transfection efficiency and high levels of transgene expression in in vitro transfection using U87 cells. Transfection efficiencies of CS/CD/TPP complex (polyplex) as well as the Lipofectamine complex (lipoplex) and Electroporation method were compared using Cherry expression plasmid. Optimum transfection efficiency and subsequent cell viability was done according to a number of experimental variables e.g. cell density, reagent and DNA concentrations, reagent-DNA complexing time, Voltage and the Pulse in electroporation methods by using Multilevel Categoric Design (General Factorial). From the results of general factorial design, it was concluded that only voltage of the exponential wave pulse has a significant influence on response. Positively charged CD/CS/TPP complexes interact with DNA by complementary electrostatic binding serve as efficient DNA delivery systems. The stability of CD/CS/TPP nanoparticle-bound DNA toward biological agents were investigated and the pmCherry-C1 was efficiently protected from DNAse I digestion.